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axiovert inverted fluorescent microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axiovert inverted fluorescent microscope
    Axiovert Inverted Fluorescent Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 2038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axiovert+inverted+fluorescence+microscope/Primovert+Compact+Inverted+Microscope/pm41792160-207-10-9
    Average 99 stars, based on 2038 article reviews
    axiovert inverted fluorescent microscope - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Electrospun Composites of Chitosan with Cerium Oxide Nanoparticles for Wound Healing Applications: Characterization and Biocompatibility Evaluation In Vitro and In Vivo.
    Article Snippet: The samples and coverslips were then fixed and stained with rhodamine-conjugated phalloidin (Thermo Fisher Scientific, Waltham, MA, USA) solution (1:500) and 4,6-diamidino2-phenylindole (DAPI, 1:40,000) (Sigma-Aldrich, Co., St. Louis, MO, USA). .. Stained cells were visualized using an Axiovert inverted fluorescence microscope (Zeiss, Oberkochen, Germany). .. Images were captured using a Canon camera (Canon Europa N.V., Amstelveen, The Netherlands).

    Article Title: Electrospun Composites of Chitosan with Cerium Oxide Nanoparticles for Wound Healing Applications: Characterization and Biocompatibility Evaluation In Vitro and In Vivo
    Article Snippet: The samples and coverslips were then fixed and stained with rhodamine-conjugated phalloidin (Thermo Fisher Scientific, Waltham, MA, USA) solution (1:500) and 4,6-diamidino-2-phenylindole (DAPI, 1:40,000) (Sigma-Aldrich, Co., St. Louis, MO, USA). .. Stained cells were visualized using an Axiovert inverted fluorescence microscope (Zeiss, Oberkochen, Germany). .. Images were captured using a Canon camera (Canon Europa N.V., Amstelveen, The Netherlands).

    Fluorescence:

    Article Title: Electrospun Composites of Chitosan with Cerium Oxide Nanoparticles for Wound Healing Applications: Characterization and Biocompatibility Evaluation In Vitro and In Vivo.
    Article Snippet: The samples and coverslips were then fixed and stained with rhodamine-conjugated phalloidin (Thermo Fisher Scientific, Waltham, MA, USA) solution (1:500) and 4,6-diamidino2-phenylindole (DAPI, 1:40,000) (Sigma-Aldrich, Co., St. Louis, MO, USA). .. Stained cells were visualized using an Axiovert inverted fluorescence microscope (Zeiss, Oberkochen, Germany). .. Images were captured using a Canon camera (Canon Europa N.V., Amstelveen, The Netherlands).

    Article Title: Tuning the Topography of Dynamic 3D Scaffolds through Functional Protein Wrinkled Coatings
    Article Snippet: .. Once 70% confluency was achieved, cells were dissociated with 0.25% trypsin for 5 min, centrifuged at 160 rcf for 5 min, resuspended with complete medium, the solution was seeded onto each sample in a 24-well plate with a density of 500,000 cells per well and 600 μL of complete medium and incubated at 37 °C/5% CO 2 for 24 h. To evaluate sample cytocompatibility, live (Calcein AM, green) and dead (Ethidium Homodimer-1, red) stains (Invitrogen, Chicago, IL, USA) were applied to the cell-seeded samples for 30 min at 37 °C prior to fluorescent imaging under an Axiovert inverted fluorescence microscope (Zeiss Microscopy, München, Germany). ..

    Article Title: Chitosan Composites with Bacterial Cellulose Nanofibers Doped with Nanosized Cerium Oxide: Characterization and Cytocompatibility Evaluation
    Article Snippet: .. An Axiovert inverted fluorescence microscope (Zeiss, Oberkochen, Germany) and a compatible Canon camera were used to visualize the cells. ..

    Article Title: Electrospun Composites of Chitosan with Cerium Oxide Nanoparticles for Wound Healing Applications: Characterization and Biocompatibility Evaluation In Vitro and In Vivo
    Article Snippet: The samples and coverslips were then fixed and stained with rhodamine-conjugated phalloidin (Thermo Fisher Scientific, Waltham, MA, USA) solution (1:500) and 4,6-diamidino-2-phenylindole (DAPI, 1:40,000) (Sigma-Aldrich, Co., St. Louis, MO, USA). .. Stained cells were visualized using an Axiovert inverted fluorescence microscope (Zeiss, Oberkochen, Germany). .. Images were captured using a Canon camera (Canon Europa N.V., Amstelveen, The Netherlands).

    Article Title: Dry and wet wrinkling of a silk fibroin biopolymer by a shape-memory material with insight into mechanical effects on secondary structures in the silk network.
    Article Snippet: .. Once 70% confluency was achieved, cells were dissociated with 0.025% trypsin for 5 min, centrifuged at 160 rcf for 5 min, resuspended with complete medium, droplet seeded onto each sample in a 96 well plate with a density of 4500 cells per well and 150 mL of complete medium, and incubated at 37 1C, 5% CO2 for 24 h. To evaluate sample cytocompatibility, live (Calcein AM, green) and dead (Ethidium Homodimer-1, red) stains (Invitrogen, IL, USA) were applied to the cell-seeded samples for 30 min at 37 1C prior to fluorescent imaging under an Axiovert inverted fluorescence microscope (Zeiss Microscopy, Germany). ..

    Article Title: AutoMorFi: Automated Whole-image Morphometry in Fiji/ImageJ for Diverse Image Analysis Needs
    Article Snippet: .. Neuronal images were captured using a Carl Zeiss Axiovert inverted fluorescence microscope with a 40X 1.3 NA oil immersion objective lens and the AxioVision Release 4.5 software. .. Images of the central brain were captured on a Nikon C2+Si confocal microscope using a 40X 1.25 NA water immersion objective lens and NIS-Elements software.

    Article Title: Measuring the biomechanical properties of cell-derived fibronectin fibrils.
    Article Snippet: CS were mounted to glass slides with fluoromount-GTM or vacuum grease; CS were mounted to glass slides with vacuum grease and pressed to approximately 1.27mm using a Mitutoyo digimatic micrometer MDC-SX. .. Immunofluorescent images were taken on a Zeiss Axiovert inverted fluorescence microscope equipped with a CCD camera (Zeiss, Oberkochen, Germany) and processed through ZEN software (Zeiss, Oberkochen, Germany). .. Optical tweezing was carried out using the dual-beam optical tweezers setup NanoTrackerTM 2 v3.0 (JPK Instruments, Berlin, Germany) assembled on a Nikon Eclipse Ti Inverted Optical Microscope platform (Nikon, Tokyo, Japan) as has previously been used to quantify forces on DNA (Gibbs et al. 2021).

    Microscopy:

    Article Title: Electrospun Composites of Chitosan with Cerium Oxide Nanoparticles for Wound Healing Applications: Characterization and Biocompatibility Evaluation In Vitro and In Vivo.
    Article Snippet: The samples and coverslips were then fixed and stained with rhodamine-conjugated phalloidin (Thermo Fisher Scientific, Waltham, MA, USA) solution (1:500) and 4,6-diamidino2-phenylindole (DAPI, 1:40,000) (Sigma-Aldrich, Co., St. Louis, MO, USA). .. Stained cells were visualized using an Axiovert inverted fluorescence microscope (Zeiss, Oberkochen, Germany). .. Images were captured using a Canon camera (Canon Europa N.V., Amstelveen, The Netherlands).

    Article Title: Tuning the Topography of Dynamic 3D Scaffolds through Functional Protein Wrinkled Coatings
    Article Snippet: .. Once 70% confluency was achieved, cells were dissociated with 0.25% trypsin for 5 min, centrifuged at 160 rcf for 5 min, resuspended with complete medium, the solution was seeded onto each sample in a 24-well plate with a density of 500,000 cells per well and 600 μL of complete medium and incubated at 37 °C/5% CO 2 for 24 h. To evaluate sample cytocompatibility, live (Calcein AM, green) and dead (Ethidium Homodimer-1, red) stains (Invitrogen, Chicago, IL, USA) were applied to the cell-seeded samples for 30 min at 37 °C prior to fluorescent imaging under an Axiovert inverted fluorescence microscope (Zeiss Microscopy, München, Germany). ..

    Article Title: Chitosan Composites with Bacterial Cellulose Nanofibers Doped with Nanosized Cerium Oxide: Characterization and Cytocompatibility Evaluation
    Article Snippet: .. An Axiovert inverted fluorescence microscope (Zeiss, Oberkochen, Germany) and a compatible Canon camera were used to visualize the cells. ..

    Article Title: Electrospun Composites of Chitosan with Cerium Oxide Nanoparticles for Wound Healing Applications: Characterization and Biocompatibility Evaluation In Vitro and In Vivo
    Article Snippet: The samples and coverslips were then fixed and stained with rhodamine-conjugated phalloidin (Thermo Fisher Scientific, Waltham, MA, USA) solution (1:500) and 4,6-diamidino-2-phenylindole (DAPI, 1:40,000) (Sigma-Aldrich, Co., St. Louis, MO, USA). .. Stained cells were visualized using an Axiovert inverted fluorescence microscope (Zeiss, Oberkochen, Germany). .. Images were captured using a Canon camera (Canon Europa N.V., Amstelveen, The Netherlands).

    Article Title: Dry and wet wrinkling of a silk fibroin biopolymer by a shape-memory material with insight into mechanical effects on secondary structures in the silk network.
    Article Snippet: .. Once 70% confluency was achieved, cells were dissociated with 0.025% trypsin for 5 min, centrifuged at 160 rcf for 5 min, resuspended with complete medium, droplet seeded onto each sample in a 96 well plate with a density of 4500 cells per well and 150 mL of complete medium, and incubated at 37 1C, 5% CO2 for 24 h. To evaluate sample cytocompatibility, live (Calcein AM, green) and dead (Ethidium Homodimer-1, red) stains (Invitrogen, IL, USA) were applied to the cell-seeded samples for 30 min at 37 1C prior to fluorescent imaging under an Axiovert inverted fluorescence microscope (Zeiss Microscopy, Germany). ..

    Article Title: AutoMorFi: Automated Whole-image Morphometry in Fiji/ImageJ for Diverse Image Analysis Needs
    Article Snippet: .. Neuronal images were captured using a Carl Zeiss Axiovert inverted fluorescence microscope with a 40X 1.3 NA oil immersion objective lens and the AxioVision Release 4.5 software. .. Images of the central brain were captured on a Nikon C2+Si confocal microscope using a 40X 1.25 NA water immersion objective lens and NIS-Elements software.

    Article Title: Measuring the biomechanical properties of cell-derived fibronectin fibrils.
    Article Snippet: CS were mounted to glass slides with fluoromount-GTM or vacuum grease; CS were mounted to glass slides with vacuum grease and pressed to approximately 1.27mm using a Mitutoyo digimatic micrometer MDC-SX. .. Immunofluorescent images were taken on a Zeiss Axiovert inverted fluorescence microscope equipped with a CCD camera (Zeiss, Oberkochen, Germany) and processed through ZEN software (Zeiss, Oberkochen, Germany). .. Optical tweezing was carried out using the dual-beam optical tweezers setup NanoTrackerTM 2 v3.0 (JPK Instruments, Berlin, Germany) assembled on a Nikon Eclipse Ti Inverted Optical Microscope platform (Nikon, Tokyo, Japan) as has previously been used to quantify forces on DNA (Gibbs et al. 2021).

    Incubation:

    Article Title: Tuning the Topography of Dynamic 3D Scaffolds through Functional Protein Wrinkled Coatings
    Article Snippet: .. Once 70% confluency was achieved, cells were dissociated with 0.25% trypsin for 5 min, centrifuged at 160 rcf for 5 min, resuspended with complete medium, the solution was seeded onto each sample in a 24-well plate with a density of 500,000 cells per well and 600 μL of complete medium and incubated at 37 °C/5% CO 2 for 24 h. To evaluate sample cytocompatibility, live (Calcein AM, green) and dead (Ethidium Homodimer-1, red) stains (Invitrogen, Chicago, IL, USA) were applied to the cell-seeded samples for 30 min at 37 °C prior to fluorescent imaging under an Axiovert inverted fluorescence microscope (Zeiss Microscopy, München, Germany). ..

    Article Title: Dry and wet wrinkling of a silk fibroin biopolymer by a shape-memory material with insight into mechanical effects on secondary structures in the silk network.
    Article Snippet: .. Once 70% confluency was achieved, cells were dissociated with 0.025% trypsin for 5 min, centrifuged at 160 rcf for 5 min, resuspended with complete medium, droplet seeded onto each sample in a 96 well plate with a density of 4500 cells per well and 150 mL of complete medium, and incubated at 37 1C, 5% CO2 for 24 h. To evaluate sample cytocompatibility, live (Calcein AM, green) and dead (Ethidium Homodimer-1, red) stains (Invitrogen, IL, USA) were applied to the cell-seeded samples for 30 min at 37 1C prior to fluorescent imaging under an Axiovert inverted fluorescence microscope (Zeiss Microscopy, Germany). ..

    Imaging:

    Article Title: Tuning the Topography of Dynamic 3D Scaffolds through Functional Protein Wrinkled Coatings
    Article Snippet: .. Once 70% confluency was achieved, cells were dissociated with 0.25% trypsin for 5 min, centrifuged at 160 rcf for 5 min, resuspended with complete medium, the solution was seeded onto each sample in a 24-well plate with a density of 500,000 cells per well and 600 μL of complete medium and incubated at 37 °C/5% CO 2 for 24 h. To evaluate sample cytocompatibility, live (Calcein AM, green) and dead (Ethidium Homodimer-1, red) stains (Invitrogen, Chicago, IL, USA) were applied to the cell-seeded samples for 30 min at 37 °C prior to fluorescent imaging under an Axiovert inverted fluorescence microscope (Zeiss Microscopy, München, Germany). ..

    Article Title: Dry and wet wrinkling of a silk fibroin biopolymer by a shape-memory material with insight into mechanical effects on secondary structures in the silk network.
    Article Snippet: .. Once 70% confluency was achieved, cells were dissociated with 0.025% trypsin for 5 min, centrifuged at 160 rcf for 5 min, resuspended with complete medium, droplet seeded onto each sample in a 96 well plate with a density of 4500 cells per well and 150 mL of complete medium, and incubated at 37 1C, 5% CO2 for 24 h. To evaluate sample cytocompatibility, live (Calcein AM, green) and dead (Ethidium Homodimer-1, red) stains (Invitrogen, IL, USA) were applied to the cell-seeded samples for 30 min at 37 1C prior to fluorescent imaging under an Axiovert inverted fluorescence microscope (Zeiss Microscopy, Germany). ..

    other:

    Article Title: 3D spheroid culturing of Astyanax mexicanus liver-derived cell lines recapitulates distinct transcriptomic and metabolic states of in vivo tissue environment
    Article Snippet: Sections were then stained in 5ug/ml WGA (InvitrogenTM, Cat.: W11261) solution in PBS containing 10ug/ml DAPI (Bittium, Cat.: 40011) for 30 min. After rinsing out unbound WGA and DAPI with PBS, samples were mounted by Prolong Gold antifade reagent (life technologies, Cat.: P36930), and subjected to imaging under a fluorescence microscope (Zeiss Axiovert inverted fluorescence microscope).

    Software:

    Article Title: AutoMorFi: Automated Whole-image Morphometry in Fiji/ImageJ for Diverse Image Analysis Needs
    Article Snippet: .. Neuronal images were captured using a Carl Zeiss Axiovert inverted fluorescence microscope with a 40X 1.3 NA oil immersion objective lens and the AxioVision Release 4.5 software. .. Images of the central brain were captured on a Nikon C2+Si confocal microscope using a 40X 1.25 NA water immersion objective lens and NIS-Elements software.

    Article Title: Measuring the biomechanical properties of cell-derived fibronectin fibrils.
    Article Snippet: CS were mounted to glass slides with fluoromount-GTM or vacuum grease; CS were mounted to glass slides with vacuum grease and pressed to approximately 1.27mm using a Mitutoyo digimatic micrometer MDC-SX. .. Immunofluorescent images were taken on a Zeiss Axiovert inverted fluorescence microscope equipped with a CCD camera (Zeiss, Oberkochen, Germany) and processed through ZEN software (Zeiss, Oberkochen, Germany). .. Optical tweezing was carried out using the dual-beam optical tweezers setup NanoTrackerTM 2 v3.0 (JPK Instruments, Berlin, Germany) assembled on a Nikon Eclipse Ti Inverted Optical Microscope platform (Nikon, Tokyo, Japan) as has previously been used to quantify forces on DNA (Gibbs et al. 2021).



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